Richa Bhardwaj
Guru Gobind Singh Indraprastha University, India
Title: Emergence of Multi Drug Resistance Escherichia coli strains against clinically significant antibiotics from river system, India
Biography
Biography: Richa Bhardwaj
Abstract
Escherichia coli is an emerging pathogen of the greatest concern as it is the leading cause of various severe infections of stomach, urinary tract, ear, wound etc. in humans. Increasing rates of antimicrobial resistance among E. coli is another furthermost fret worldwide. This problem is more traumatic when water bodies are getting contaminated with faecal pollution and inappropriate use of antibiotics that led to emergence of multi-drug resistant strains of this normal microbiota of human intestine. The current study dealt with the isolation of twenty-eight E. coli strains from water samples collected from River Yamuna in Delhi stretch of India. E. coli was tested for antibiotic susceptibility by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standard Institute (CLSI) guidelines using 24 different antibiotics belonging to three different modes of action namely β-lactams, aminoglycosides, phenicols, tetracyclines and quinolones. Most evident finding of the study was that none of antibiotic used in the study was 100% effective. In total, 100% of the isolates exhibited multi drug resistance (MDR) character and all the isolates had a very high multiple antibiotic-resistance (MAR) index, suggesting the origin of the isolates to be of high antibiotic usage. MAR index for all the isolates were calculated on the basis of resistance patterns. It has been observed all the strains were having the MAR value > 0.2 and up to 1, showing very high degree of multi-drug resistance. Therefore, the analysis is highly informative in terms of assessing the faecal contamination of river water, determining resistance of E. coli against the commonly available significant antibiotics and prediction of future emergence of MDR strains.
