Woo-Jung
Gyeonggi Institute of Science and Technology Promotion (GSTEP) South korea
Title: Liquid Chromatography-Mass Spectrometry-based Rapid Secondary-Metabolite Profiling of Marine Pseudoalteromonas sp. M2
Biography
Biography: Woo-Jung
Abstract
The ocean is a rich resource of flora, fauna, and food. A wild-type bacterial strain showing confluent growth on marine agar with antibacterial activity was isolated from marine water, identified using 16S rDNA sequence analysis as Pseudoalteromonas sp., and was designated as strain M2. This strain was found to produce various secondary metabolites including quinolone alkaloids. Using high-resolution mass spectrometry (MS) and nuclear magnetic resonance (NMR) analysis, we identified 9 secondary metabolites of 4-hydroxy-2-alkylquinoline (pseudane-III, IV, V, VI, VII, VIII, IX, X, and XI). Additionally, this strain produced 2 novel closely related compounds, 2-isopentylqunoline-4-one and 2-(2,3-dimetylbutyl)qunoline-4-(1H)-one, which have not been previously reported from marine bacteria. From the metabolites produced by Pseudoalteromonas sp. M2, 2-(2,3-dimethylbutyl)quinolin-4-one, pseudane-VI, and pseudane-VII inhibited melanin synthesis in melan-a cells by 23.0%, 28.2%, and 42.7%, respectively, wherein pseudane-VII showed highest inhibition at 8 µg/mL. The results of this study suggest that liquid chromatography (LC)-MS/MS-based metabolite screening effectively improves efficiency of novel metabolite discovery. Additionally, these compounds are promising candidates for further bioactivity development.