Fanyana Mtunzi
Vaal University of Technology, South Africa
Title: Evaluation of biological activities of nine medicinal plants and isolation and identification of antimicrobial compounds from Pomaria sandersonii and Alepidea amatymbica
Biography
Biography: Fanyana Mtunzi
Abstract
limit. Carissa bispinosa hexane fraction displayed the lowest activity of 467.7 mg/L within 264.0 to 828.5 at 95% confidence limit. Highest ABTSâ—+ radical scavenging was demonstrated by Pomaria sandersonii DCM, (1.273 mg/L at 0.9439 to 1.719 within 95% confidence limit) for the Ethyl acetate, (5.973 mg/L at 4.722 to 7.555 within 95% confidence limit while the lowest activity was displayed by hexane fraction from Eucomis autumnalis (929.4 mg/L at 557.5 to 1550 within 95% confidence limit). The activity of Pomaria sandersonii extracts and fractions demonstrated that the plant contains antioxidants that react with both DPPH and ABTS radicals although higher activities were shown by ABTS as displayed by the lower EC50 values. All of the crude fractions and extracts displayed high to moderate anti-bacterial activity (20-625 µg/ml) and anti-fungal activity (20-2500 µg/ml). P. sandersonii crude and fractions demonstrated highest antimicrobial activity compared to other plants. Some MIC values P. sandersonii DCM and ethyl acetate (80 µg per ml in each case) compared well with gentamycin (4 µg per ml) same value against S. aureus, E. faecalis, E. coli and P. aeruginosa. Anti-fungal activities of the DCM, acetone and methanol fractions were also highly active (20 µg per ml) for both Candida albicans (C. a.) and Aspergillus. fumigatus (A. f.) Inhibition of pathogen growth demonstrated by the polar fractions indicates that most of the active compounds would be soluble in water. P. prunelloides, crude methanol extract produced 60% sucrose component and G. perpensa, 36% was glucose. Three compounds isolated from Alepidea amatymbica and three from Pomaria sandersonii which displayed high antimicrobial activities.