Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series : World's leading Event Organizer

Back

Yuan Liu

Yuan Liu

Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, China

Title: Long-term application of farmyard manure to arable soils does not, in itself, promote the spread of antibiotic resistance genes

Biography

Biography: Yuan Liu

Abstract

Misuse of antibiotics has exacerbated the development and spread of antibiotic resistance in microbes in the environment, promulgating calls for nations to adopt mitigation strategies. Farmyard manure (FYM) has a long history in soil fertility management which is being re-evaluated because of a combination of rising mineral fertilizer costs, concerns for sustainable soil management and ecological stability. However, FYM may act as a reservoir of antibiotic resistance genes (ARGs) and resistant organisms: the effect of long-term application of FYM on ARGs in soil needs to be established. We studied ARGs in arable soil treated for 175-years with FYM or chemical fertilizer (CF) and native woodland (W). Metagenomic analysis showed that the abundance of fox5, blaCTX-M-4, vgb, tetG, sul1, vanA and floR genes were significantly lower in FYM than W, and the abundance of fox5, vgb and tetX genes in FYM were also significantly lower than CF. The phylogenetic diversity of individual ARGs was also typically lower in FYM than either CF and W. Despite this, there were no significant differences in the abundance of integrase genes (intI1 and intI2). There were also no significant differences in the abundance of metal resistance genes, except merB associated with Hg-resistance, which was more abundant and had an increased phylogenetic diversity in FYM soils. Overall, FYM did not increase the abundance and diversity of ARGs significantly and there was only limited association between ARGs and metal resistance genes in the soils.