9^th World Congress and Exhibition on Antibiotics and Antibiotic Resistance March 07-08, 2022 Barcelona, Spain

Biography:

Abstract:

Urinary tract infection (UTI) is one of the most common bacterial infections and is a major public health problem. The objective of this work is to propose, based on updated data, the microbial ecology of UTI at the Avicenne Military Hospital in Marrakech, and to monitor the antibiotic sensitivity profile of uropathogenic bacteria.This work is a retrospective study with a descriptive aim, which was carried out on 21,407 cytobacteriological urinary examinations of inpatients and outpatients treated at the microbiology laboratory of the Avicenne military hospital in Marrakech, over a 6-year period from January 2014 to December 2020. The cyto-bacteriological examinations are carried out using conventional or automated techniques. The culture is done on agar medium. The antibiogram is done by automated technique (the Phoenix® 50 from BectonDeckinson) and the interpretative reading of the antibiogram is done according to the recommendations of EUCAST (European Committee on Antimicrobial Susceptibility Testing). The statistical analysis of the results is done on SPSS

Biography:

I hereby take this opportunity to introduce myself as Dr. Jeet Bahadur Moktan, Pharm.D graduate (graduated in 30th of June 2017) from India.

I have good academic background. My internship profile includes the knowledge in “Drugs and pharmaceutical care”. I have been currently working as a Research Scholar at Adichunchanagiri University and Clinical Coordinator at Clinical Trials Centre, AH&RC, ACU.

Abstract:

Antibiotics are one of our most effective weapons in the fight against life-threatening infections. Antibiotic resistance does not indicate that the body has developed tolerance to antibiotics; rather, microorganisms have developed resistance to the drugs intended to kill them. Methods: This study was prospective and observational and conducted in inpatients in various wards of AH&RC. Objective: To identify and evaluate the antibiotic resistance. Results: After the informed consent process a total of 200 samples (5 ml of blood and 5- 10 ml of urine) from 200 different patients were collected and cultured. The mean age of the patients was 37.12 years (range 0 - 91) and 109 (54.5%) were males and 91 (45.5%) females. Bacterial pathogens were detected in 56 samples, 25% of which were the maximum from under 20 years age group and least was from the 70 years and older. In the study we found more GNB compared to the GPB. In case of GNB we found 39(19.5%) followed by GPB 17(8.5%) and remaining 144(72%) of samples showed no growth. Overall, E. coli isolates were highly resistant to ampicillin (100%) followed by ceftriaxone 84.21%, ceftazidime 78.94%, and ciprofloxacin 76.19%. Nitrofurantoin (15.78%) demonstrated the lowest rate of resistance in the study, in case of E. coli. Conclusion: Bacteria that cause antibiotic resistance can infect anyone, at any age, in any nation. They result in longer hospital stays, more medical costs, and more deaths that could have been avoided. Antibiotic resistance is one of the most serious dangers to world health, according to the WHO. As a result, as humans, we must support the healthcare team by adhering to guidelines or protocol at all levels by (i) increasing awareness and understanding of AMR through effective communication, education, and training; and (ii) strengthening knowledge and evidence through surveillance. Key words: antibiotic, antibiotic resistance, microorganisms, infections, community.

Biography:

Luca Scotti has completed his PhD from University of Chieti-Pescara, Italy and BSc. Chemistry at University of Milano, Italy. He is the Professor of Biochemistry at department of Medical, Oral and biotechnology (DISMOB), Italy. He has over 30 publications that have been cited over 300 times, and his publication h-index is 11. He has been serving as an editorial board member and topics member of several reputed journals.

Abstract:

This work highlights how our silver ultra nanoclusters (ARGIRIUM‐SUNc) hand‐made synthesized, are very useful as a bactericide and anti‐biofilm agent. The Argirium‐SUNc effective antibacterial concentrations are very low (< 1 ppm) as compared to the corresponding values reported in the literature. Different bacterial defense mechanisms are observed dependent on ARGIRIUM‐SUNc concentrations. Biochemical investigations (volatilome) have been performed to understand the pathways involved in cell death. By using fluorescence techniques and cell viability measurements we show, for the first time, that membrane depolarization and calcium intracellular level are both primary events in bacteria death. The ARGIRIUM‐SUNc determined eradication of different biofilm at a concentration as low as 0.6 ppm. This suggests that the effect of the nanoparticles follows a common mechanism in different bacteria. It is highly probable that the chemical constitution of the crosslinks could be a key target in the disrupting mechanism of our nanoparticles. Since the biofilms and their constituents are essential for bacterial survival in contact with humans, the silver nanoparticles represent a logical target for new antibacterial treatments.